Coding

Part:BBa_K2288001:Design

Designed by: Jiawen Yang   Group: iGEM17_NEU-China   (2017-10-10)


Rho-OR1D2(HEK293)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 28
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 345


Design Notes

The annealing temperature needs to be set at 48-50 degree during PCR.


Source

This part comes from the genomic sequence of 293FT.

References